ABSTRACT
Objective To explore the value of intravoxel incoherent motion (IVIM) analysis in evaluation of pathological complete response (pCR) in patients with locally advanced rectal cancer (LARC) after neoadjuvant chemotherapy.Methods Totally 61 patients with pathologically proven rectal adenocarcinoma were retrospectively analyzed.All patients underwent MR scan,including multi-b DWI IVIM sequence scanning before and after neoadjuvant chemotherapy.ADC values and IVIM parameters (slow diffusion coefficient [D],fast diffusion coefficient [D*] and perfusion fraction [f]) were measured by two radiologists independently.Then the patients were divided into pCR group (n=14) and non-pCR group (n=47).Independent-samples t test was used to compare all the parameters before and after therapy,as well as the percentage changes (△ratio) between pCR and non-pCR group.Area under the curve (AUC) was used to evaluate the diagnostic performance.Results ADC (P=0.002) and D (P=0.007) values were significantly lower before therapy,while △ratio ADC (P＜0.001) and △ratio D (P =0.002) were significantly higher in pCR group compared with those in non-pCR group.D*,f and their △ratio value were not statistically different pre-chemotherapy and post-chemotherapy in both two groups (all P＞0.05).△ratio D had the highest AUC (0.786,P＜0.01).Conclusion IVIM-derived D values can help to predict and differentiate pCR of LARC after neoadjuvant chemotherapy.
ABSTRACT
Objective To compare the epidermal shape built by different passages of keratinocytes and its ability of proliferation and differentiation in three-dimensional conditions.Methods Different passages of keratinocytes were used to construct tissue-engineered skin.The morphology of the tissue-engineered skin was observed with HE and PAS staining,while CK1/CK10,CK5/CK14,Ki67 were detected by immunohistochemical assays.Results All the tissue-engineered skin had a significant dermoepidermal structure.The stratification of 1st and 2nd passage skins were better,and 2nd passage epidermis was thicker than that in other passages (P＜0.05).Dermoepidermal structure in collagen type Ⅳ group binded more tightly,but collagen type Ⅳ had little effect on the thickness of the epidermis (P＞0.05).In collagen type Ⅳ group PAS stain was negative,indicating type Ⅳ collagen was unable to promote the reconstruction of BM in vitro.The Ki-67 proliferation index of the 2nd keratinocyte was similar to the normal skin,the remaining passages keratinocyte proliferation gradually decreased (P＜0.05) ; the 1st and 2nd passage skins expressed CK1/CK10 and CK5/CK14.Conclusions Keratinocytes before the 3rd passage have a better ability in the proliferation and differentiation,and so they are more suitable as seed cells for tissue-engineered skin.
ABSTRACT
Objective To evaluate the clinical characterization and therapy efficacy on early epilepsy patients after viral encephalitis.Methods To analyze eighty-two early epilepsy patients after viral encephalitis about their onset of illness age,epileptic type,infarction locus and therapy efficacy.Results Early epilepsy patients after viral encephalitis patient's condition and incidence is associated with severity of viral encephalitis and complication.Conclusion To ef- fectively control the patient's condition of viral encephalitis and complication is associated with onset of epilepsy.